Heat stress responses to increasing temperature humidity index (THI) in lactating Murrah buffalo

The present study was conducted to determine the effect of increasing temperature humidity index (THI) on lactating Murrah buffalo. six buffaloes which were offered same basal diet and blocked by days in milk, milk yield parity. during May, June July with average THI 79.88, 80.57 85.36, respectively. Milk recorded twice a day at 06:00 18:00 h physiological parameters blood sampled 30th each month 14:30 h. results showed significant decrease (P<0.05) only 85.36 as compared up 80.57. rectal (RT) did not change (P=0.580) however, respiration rate (RR) pulse (PR) changed progressively THI. Leucocytopnoea, lymphocytopnoea neutrophilia; alteration serum urea, alkaline phosphatase activity, reactive oxygen species phosphorus concentration observed Similarly, T4, cortisol prolactin levels It can be concluded that experienced mild heat stress (May June) moderate type (July).